fa تحقيقي Original Paper <div><strong>ABSTRACT</strong><br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Three major hepatitis B virus (HBV) antigens include HBcAg, HBeAg and HBsAg. HBeAg is the extracellular form of HBcAg, and is seen almost exclusively in people who have circulating serum HBV DNA. Presence of HBsAg in serum indicates that the individual has contracted HBV infection. Chronic hepatitis HBeAg-negative/anti-HBe&ndash;positive is known as an important form of chronic hepatitis B in the Mediterranean region. In this report, we used Real-Time PCR and ELISA for detection of HBV and HBeAg/HbsAg, respectively. In our investigation on 4743 HBV cases referred to the Mahdieh Clinical Laboratory between 2008 and 2016, we found a <a name="OLE_LINK17"></a><a name="OLE_LINK16">53-year-old man with clinical symptoms of hepatitis and abnormal molecular and serological features</a>. Despite the presence of clinical symptoms and high viral load (128 &times; 10⁵iu/ml), the patient was HBsAg-positive and HBeAg-negative. Identifying this type of HBV could indicate spread of this type of hepatitis in Isfahan, Iran.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords: </strong>Hepatitis B, HbsAg, HBeAg.</div> Hepatitis B, HbsAg, HBeAg. 33 35 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-483&slc_lang=fa&sid=1 Majid Komijani 100319475328460013910 100319475328460013910 No Department of Biology, Faculty of Science, Arak University, Arak, Iran Mohammad Taghi Kardi m.kardi@gmail.com 100319475328460013911 100319475328460013911 Yes Department of Microbiology, Mahdieh Laboratory, Isfahan, Iran Khashayar Shahin 100319475328460013912 100319475328460013912 No State Key Laboratory Cultivation Base of Most, Institute of Food Safety and Nutrition, Jiangusa Academy of Agricultural Sciences, Nanjing 210014,PR China Mahsa Yazdi 100319475328460013913 100319475328460013913 No Department of Biology, Faculty of Sciences, University of Isfahan, 81746073441 Isfahan, IR Iran