Medical Laboratory Journal

en Comparing the cytotoxic effects of iron oxide nanoparticles synthesized using the cytoplasmic extract of Lactobacillus casei and chemical synthesis دیگر Others Research Article Research Article <div style="text-align: justify;">Background: Discovering new cytotoxic compounds has received significant attention due to the rise in drug resistance and the adverse effects associated with chemotherapy drugs. In this study, the cytoplasmic extract of Lactobacillus casei was used to produce iron oxide nanoparticles (Fe2o3 NPs), and the cytotoxic effects of NPs were investigated on MCF-7 and human embryonic kidney 293 (HEK-293) cells. Methods: The cytoplasmic extract of L. casei was mixed with 103M iron sulfate solution and incubated for 3 weeks at 37 °C and 5% CO2. The coprecipitation method was used to synthesize chemical Fe2o3 NPs. The synthesis of NPs was studied by electron microscopy and X-ray diffraction (XRD) analysis, and the cytotoxic effects were evaluated with dilutions (10, 100, and 1000 µg/mL) on MCF-7 and HEK cells. Results: X-ray diffraction analysis and scanning electron microscopy presented the mean of NPs synthesized by the green method to be about 15 nm and their shape to be spherical, as well as the average of chemically synthesized NPs to be about 20 nm with cubic structure. Chemical and green synthesized NPs only at a concentration of 1000 µg/mL were able to significantly reduce the survival rate of normal HEK-293 cells; chemically synthesized NPs decreased MCF-7 cell survival only at 1000 µg/mL and green synthesis at 100 µg/mL and 1000 µg/mL. Conclusion: Generating Fe2o3 NPs is biologically safe using the green synthesis method and the cytoplasmic extract of L. casei, which may be a suitable candidate for the treatment of cancer cells.</div> Magnetic iron oxide nanoparticles,Lactobacillus casei,MCF-7 cells,HEK293 cells 16 18 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1092-1&slc_lang=en&sid=1 mahnoosh fatemi 100319475328460032025 100319475328460032025 No Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Fereshte Ghandehari fe_gh_2010@yahoo.com 100319475328460032026 100319475328460032026 Yes Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Danial Salehi 100319475328460032027 100319475328460032027 No Department of Chemical Engineering, Isfahan University of Technology, Isfahan, Iran parastoo Torabian 100319475328460032028 0009-0002-7522-4714 No Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran