en قارچ شناسی Mycology Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> The reference method for antifungal susceptibility testing is broth microdilution according to Clinical and Laboratory Standard Institute (CLSI) guidelines. However, the fully automated system, Vitek 2C system may reduce the workload and observer bias associated with manual broth microdilution. This study aimed to compare the results of YS08 card with the results of the broth microdilution (BMD) method.<br> <b>Methods:</b> A total of 50 clinical Candida isolates were included in the study. The susceptibility testing was done by Vitek 2C using the YS08 card. Broth microdilution was done according to CLSI guidelines M27M44S-Ed3.<br> <b>Results:</b> For C. albicans, the categorical agreement was 85.8%, 71.5%, 85.8%, and 100% for fluconazole, voriconazole, caspofungin, and micafungin, respectively. The minor errors (MiE) of 14.2% for fluconazole and caspofungin, 28.5% for voriconazole, were detected in C. albicans. In C. glabrata, the categorical agreement (CA) was 100% for micafungin, voriconazole, but 63.7% for caspofungin. An MiE of 36.3% was detected for caspofungin. C. parapsilosis showed a 100% CA for fluconazole, caspofungin, and micafungin, and 85.8% for voriconazole. There were 14.2% of MiEs for voriconazole. In C. tropicalis, 100 % CA was observed for fluconazole, micafungin, and caspofungin, and 88.9% for voriconazole. Moreover, 11.1% (1/9) of MiEs was observed for voriconazole. In C. auris, there was a 100% CA for caspofungin and micafungin, 77.8% for fluconazole, and 66.7% for amphotericin B. There was a major error of 22.2% for fluconazole and 33.3% for amphotericin B.<br> <b>Conclusion</b>: The majority of Vitek 2C showed comparable results with the broth microdilution (BMD) method. Only minor errors (MiEs) were observed in the tested Candida species.</span></span></div> Antifungal susceptibility testing, Candida, Broth microdilution 4 7 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1456-1&slc_lang=en&sid=1 Sukanya Sudhaharan sukanyavimala@gmail.com 100319475328460029204 100319475328460029204 Yes Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India Naga Appala Raju Sundarapu sundarapu223@gmail.com 100319475328460029205 100319475328460029205 No Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India Umabala Pamidimukkala umapamidi@gmail.com 100319475328460029206 100319475328460029206 No Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India